Zwitterionic and pI Balancing Cy Dyes
Two-dimensional difference gel electrophoresis (2D-DIGE) has been an established technique within the proteomics field. Current standardCyDyes utilized for protein labeling for 2D-DIGE experiments have had limitations, however, leading to decreased water solubility, protein precipitation, and low sensitivity. Researchers at Montana State Universityhave recently synthesized a new set of fluorescent dyes, known as zwitterionic CyDye fluorophores (Z-CyDyes), that have shown to be improved versions of the commercially available CyDyes for protein amine labeling.
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